Meagher Laboratory

 

Arabidopsis has six nuclear ARPs with known or likley roles in chromatin remodeling: AtARP4, AtARP5, AtARP6, AtARP7, AtARP8, and AtARP9
 

The localization of ARP7 (green monoclonal antibody localization) and acetylated histone H2B (red antibody localization) during the cell cycle in Arabidopsis root cells shows that ARP7 is released from chromatin during metaphase and telophase, while a typical histone remains bound. DNA is stained blue with DAPI.

 

 

 

 

 

 
This page shows the nuclear localization pattern for ARP7. We have shown that the nuclear localization of ARP4 and ARP6 is quite similar. All cells and tissues appear to express most of the nuclear ARPs, although their levels vary widely among different tissues and organs.

 

These two photos were taken of the same strip of root apical cells stained with antibody (green), which localizes AtARP7 protein to the nucleoplasm, and co-stained with DAPI (red), which localizes DNA. Hence AtARP7 is localized in the same cellular location with DNA. The cytoplasm of these cells stain weakly for AtARP7, but not for DNA. ARP4, 6, and 8 have also been localized to the nucleus.

 

Nuclear localization of Arabidopsis ARP4. Arabidopsis ARP4 is localized to interphase nucleoplasm, but is cytoplasmic in dividing cells lacking a nuclear membrane.