Meagher Laboratory

Constitutive and reproductive profilin expression patterns parallel vegetative and reproductive actins

     
Profilin expression in Arabidopsis root callus
Profilin expression in Arabidopsis pollen tubes
 
Expression of constitutive (vegetative) profilin 1 (PRF1) is detected in root tissue but not in pollen tube with a specific mouse monoclonal antibody (PRFmAb1a). Expression of pollen specific profilins 4 and 5 (PRF4, PRF5) is detected inpollen tube but not root tissue with a specific mouse monoclonal antibody (PRFmAb45a).
Expression of all five Arabidopsis profilins is detected in both root tissue and pollen tubes with a polyclonal rabbit antibody PRFpAb that reacts with all plant profilins
   
Kandasamy et al., 2002b